Perspective - (2023) Volume 9, Issue 2

The Chemical Ligation is A Fundamental Technique in Genetic Engineering and Recombinant DNA Technology
Benedikt Schober*
Department of Radioligands, Jagiellonian University, Poland
*Correspondence: Benedikt Schober, Department of Radioligands, Jagiellonian University, Poland, Email:

Received: 31-May-2023, Manuscript No. jbcc-23-101091; Editor assigned: 02-Jun-2023, Pre QC No. jbcc-23-101091; Reviewed: 16-Jun-2023, QC No. jbcc-23-101091; Revised: 21-Jun-2023, Manuscript No. jbcc-23-101091; Published: 28-Jun-2023, DOI: 10.33980/jbcc.2023.v09i02.0015


The chemical ligation of unprotected peptide segments is facilitated by the formation of a non- native bond at the ligation site, which allows for chemo selective condensation. Most of the time, chemical ligation is done in an aqueous solution. Proteins of the typical size found in nature, i.e. with polypeptide chains containing 200-300 amino acids, produced by total synthesis, can be produced through a series of chemical ligation reactions. It comprised of a clever way to deal with the covalent build-up of unprotected peptide portions through special, commonly receptive functionalities, one on each responding peptide fragment, intended to respond just with one another and not with any of the practical gatherings tracked down in local peptides.


The formation of an unnatural moiety or non-peptide bond that connects the two peptide segments in the ligation product makes chemical ligation of unprotected peptides possible. It was envisioned as a general strategy that would make the chemical synthesis of protein molecules much simpler and make it possible to apply the entire chemistry repertoire to the world of proteins. As an alternative to native chemical ligation, Ser/Thr ligation was incorporated into protein chemical synthesis. Condensation of a side-chain unprotected peptide segment with a C-terminal salicylaldehyde ester and another peptide segment with an N-terminal Ser/Thr residue is known as serine/threonine ligation. An N, O-benzylidene acetal-linked intermediate is formed during the chemo selective reaction between the peptide salicylaldehyde ester and the 1, 2-hydroxylamine group of Ser or Thr. This intermediate undergoes acidosis to provide a natural peptide Xaa-Ser/Thr linkage. Ser/Thr ligation gives a correlative strategy to protein compound union and semi blend. In native chemical ligation, two unprotected peptides undergo a chemo selective reaction in an aqueous solution to produce a single covalently linked ligation product. In biology, the enzymatic reaction that forms a covalent bond between two biomolecules is referred to as ligation, which can be defined as the act of joining. The use of DNA ligation in molecular biology research is explained in this video. At present the three most normal ligation frameworks are dynamic and detached self-ligation and customary flexible ligation. The three moves toward structure a new phosphodiester bond during ligation are: Nick sealing, adenylyl transfer to DNA, and enzyme adenylylation. A fundamental method in recombinant DNA technology and genetic engineering is the ligation, or joining of two or more DNA fragments by means of a reaction mediated by ligase. New functional units can be created through the ligation of various DNA fragments. A "block condensation" method for copying genetic information is the non-enzymatic ligation of RNA strands on a template. Chemical ligation is an enzyme- free method for synthesizing longer RNA constructs if performed with a short splint strand instead of a long template.


As a result, there are at least three possible ligations: vector self-ligation, insert self-ligation, and vector- insert ligation. In organic chemistry, a ligase is a protein that can catalyse the joining of two particles by shaping another synthetic bond. Ligase is an enzyme that helps two molecules bind together. A DNA ligase, for instance, uses phosphodiester bonds to connect two DNA fragments.

Copyright: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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