The present study is carried out with the lactic acid bacteria isolation and identification such as Lacto-bacillus fermentum from curd sample. Five isolates ofLactobacilli supernatant is examined for acid and antibacterial activityproduction. The extracted compound showed antibacterial activityagainst the microorganisms. Lactobacil-lus fermentum showed better antimicrobial activity. Antibacterial activitiesLABaredietary supplements of live microorganisms thought to be healthy for the host organisms. In this study, lactic acid bacteria are isolated from 2 different curd samples. A total of 20 strains are studied for their characterization, from that 2 potential strains Lac-tobacillus acidophilus and Lactobacillus plantarum isolated from curd. The potential strains are studied by curd pro-duction, antibacterial and bactericidal activity also their antibiotic susceptibility. The quality of curd is analyzed by qualitatively and quantitatively by nutrient evaluation. The antibiotic resistance of potential strain is studied using the good resistance activity to the antibiotics. The curd produced by those strains has good quality and quantityof nutrients.