Nanotechnology is an emerging interdisciplinary technology that has been used in many areas in the last decades. A Zinc Oxide nanoparticle (ZnONPsNP’s) has a great importance due to different properties like anti-wrinkle, anti-microbial, fire retarded etc. It can be used for the various applications in textiles. ZnONPsNP’s can be synthesized by various methods like wet chemical methods, sol-gel method, co-precipitation and biological methods etc. In this work ZnONPsNP’s were synthesized by wet chemical method. Zinc acetate (0.1M) and potassium hy-droxide (0.1M) are mixed together in presence of 50% ethanol at room temperature give precipitates and filtered. The precipitated was then heated in muffle furnace at 300oC to for 3-4h. Different concentration of Ethanol was used as a solvent for homogeneity of the solution andit help to make a stoichiometric solution in order to obtain ZnONPsNP’s. As obtained ZnONPsNP’s are characterized Using Ultraviolet visible spectroscopy (UV-Vis), Particles size analyzer (CPS), X-ray Diffraction (XRD). The antimicrobial activity of ZnONPsNP’s was carried out using agar-well diffusion method. The results shows that UV-Vis absorption wavelength 365nm,CPS shows the average particles size 88nm and XRD data gives the crystalline structure of ZnONPsNP’s. The antimicrobial activity against Gram positive and gram negative organisms was found 17mm and 10mm respectively. Theses ZnONPsNP’s were used for rayon textiles applications.