A Schiff base and its Pd(II), Pt(II) complexes have been synthesized. The characterization of ligand p-methoxy isonitroso acetophenone (p-MINAP), Pd(II) and Pt(II) complexes have been studied by elemental analysis, molar conductance, magnetic susceptibility, Electronic, Infrared, 1H NMR spectroscopy. On the basis of molecular weight, magnetic susceptibility and spectral data, octahedral geometry has been assigned to Pd(II) and Pt(II) com-plexes. Antimicrobialscreening of Pd(II) and Pt(II) was carried out with p-methoxy isonitroso acetophenone(p-MINAP). This shows potent biological activity against gram positive bacteria B. cereus, B. subtillis, S. aureus and gram negative bacteria E. coli, K. pneumonae, P. aeruginosa and fungi A. niger, F. oxysporium, C. albicans by usingagar dilution method.