The in vitro studies on physical and nutritional requirements (Carbon and Nitrogen sources) for the growth and sclerotial production in Morchella hybrida(Sow.) Pers. have revealed that this morel grows and repro-duces best at an optimum temperature of 24 ??C and pH 7.0 after ten days of incubation on Czapek’s medium. The growth and sclerotia production on nine different carbon compounds viz.D(+)xylose, D(+)glucose, D(-)fructose, L(-)sorbose, Maltose, Lactose, Sucrose, Raffinose and Starch was investigated. Out of these nine carbon sources, D(+)xylose have been found to be the best for growth and sclerotia production. Twenty six different nitrogen com-pounds viz.Potassium nitrate, Sodium nitrate, Sodium nitrite, Ammonium acetate, Ammonium oxalate, Ammonium chloride, Ammonium sulphate, Ammonium phosphate, Ammonium nitrate, L-arganine, L-cysteine HCl, DL-aspartic acid, L-cysteine, L-glutamic acid, Glycine, L-histidine HCl, L-leucine, DL-methionine, L-ornithine HCl, L-β-phenylalanine, L-proline, DL-serine, L-tyrosine, DL-valine, DL-tryptophan and DL-threonine have been used for the nitrogen nutrition studies. Out of these twenty six nitrogen sources, the fungus produces maximum mycelial growth with L-glutamic acid and the best production of sclerotia with L-glutamic acid and L-ornithine HCl.