Bio-Indicator Lichens of Sikandra Hills of North West Himalaya

Monika Thakur1 and Hem Chander2*

1 & 2 Division Botany, Department of Bio-Sciences, Career Point University Hamirpur, (H.P.), INDIA

* Correspondence: E-mail: hemchander78@gmail.com

(Received 11 Oct, 2018; Accepted 09 Nov, 2018; Published 20 Nov, 2018)

ABSTRACT: During the lichen floristic studies, three hundred specimens of lichens were collected from Sikandra hill, which is situated in Shivalik zone of North West Himalaya. These specimens were then investigated morpho-chemo-taxonomically and thirty species of lichens have been identified. Out of these, ten species of lichens (viz. Candelaria concolor (Dicks.) Arnold,Heterodermia pseudospeciosa (Kurok.) W.L. Culb,Lecanora chlarotera Nyl,Parmotrema praesorediosum (Nyl.) Hale,Parmotrema tinctorum (Despr. ex Nyl.) Hale,Phaeophyscia hispidula (Ach.) Essl, Physcia stellaris (L.) Nyl., Punctelia subrudecta (Nyl.) Krog, Pyxine subcinerea Stirt) act as bio-indicator. Candelaria concoloris a nitrophile and act as indicator of nitrogen pollution, whereas,Punctelia subrudecta is nitrogen tolerant. Heterodermia pseudospeciosa belong to physcioid lichen community and is toxi-tolerant species. The other seven lichen speciesact as bio-indicator of heavy metal air pollutants (iron, chromium, copper, zinc, lead and nickel. These potential bio-indicator lichen species can be used for monitoring of environmental quality in the study area.

Keywords: Bio-indicator; Heavy metal; Nitrophile; Pollutant and Toxi-tolerant.

INTRODUCTION: Lichens are bio-indicators of air pollution, especially sulfur dioxide pollution. They are inexpensive to use in evaluating air pollution and are able to react to wide range of air pollutants over a period of time as compared with other physical/chemical monitors. The quality of environment in a particular area can be assessed either by monitoring changes in lichen community or through monitoring their physiological changes. The toxic elemental pollutants and radioactive metals bind with mycobiont and concentrate over time. Lichens were recognized as potential indicators of air pollution as early as the 1860's in Britain and Europe1. Since then, lichens have played prominent role in air pollution studies throughout the world because of their sensitivity to different gaseous pollutants, particularly sulfur dioxide. They have also been found to act as accumulators of elements, such as trace metals, sulfur, and radioactive elements2-3. The lichen species best suited as bio-monitors are foliose (having a lobed, leaf-like shape) and fruticose (having upright or pendulous branches) epiphytic lichens. The properties that make them suitable for monitoring purposes are the weakly developed cuticle and vascular bundles, absence of real roots, their slow growing nature and prolonged life cycle and their broad distribution 4. Indicators are required to monitor ecological conditions of habitats. Lichens have been found to be very much sensitive to environmental parameters like temperature, humidity, wind and air pollutants because they don’t have any vascular system and thus absorb water and nutrients passively from their surrounding environment. Lichen species composition and changes in composition is a very powerful tool to get information about changes in climate, air quality and biological processes. The lichens respond to the environmental changes by reflecting changes in their diversity, abundance, morphology, physiology, accumulation of pollutants etc. The main threats such as habitat degradation and loss, habitat fragmentation, overexploitation, air pollution, and climate change which affect biodiversity in general are also applicable for lichens 5. Lichens are also measurable. Due to these unique features, lichens may be used as relevant indicators for ecosystem productivity and biodiversity. Biological monitoring using lichen as indicator may be considered a very effective tool for early warning system to monitor and detect climate change and air pollution 6. The unregulated harvesting of lichens has become a serious hazard to biodiversity in Himalayas and Western Ghats7. Lichens are very important for nutrient cycling 8. Lichens absorb air and rain-borne nutrients for their use and thus contribute in ecosystems cycling. Some lichen species help in fixing nitrogen through a symbiotic relationship with cyanobacteria which contribute good amount of nitrogen to forest ecosystems 9-12.

MATERIALS AND METHODS : During the present study, lichen specimens were collected from in and around Sikandra Dhar. Sikandra Dhar is situated in Shivalik hill zone of North Western Himalaya and is located in district Mandi of Himachal Pradesh (India). The study area spreads in Suket, Bhambla and Nagrota forests. The specimens were collected from various habitats and substrates. The field data such as texture, size, colour, macroscopic features and form have been noted in the field book during the excursions 13. All the specimens have been preserved in CPUH (The Herbarium, Department of Bio-Sciences, Career Point University, Hamirpur). The collected lichen specimens were initially segregated according to their growth forms. Within the growth forms the specimens were further grouped according to the type of fruiting bodies (apothecia, perithecia, sterile). The lichens were identified by studying their morphology, anatomy and chemistry. Authenticated taxonomic keys were referred for identification of lichen specimens 14-16. The chemicals used for the chemical spot tests of the lichens were prepared using standard method 17.

RESULTS AND DISCUSSION: On the basis of morpho-chemo-taxonomic investigations, a total of twenty five species of lichens were identified.18 Out of these, ten species of lichens (viz. Candelaria concolor (Dicks.) Arnold, Heterodermia pseudospeciosa (Kurok.) W.L. Culb, Lecanora chlarotera Nyl,Parmotrema praesorediosum (Nyl.) Hale,Parmotrema tinctorum (Despr. ex Nyl.) Hale, Phaeophyscia hispidula (Ach.) Essl, Physcia stellaris (L.) Nyl., Punctelia subrudecta (Nyl.) Krog,Pyxine subcinerea Stirt) act as bio-indicator. Candelaria concoloris a nitrophile and act as indicator of nitrogen pollution, whereas, Punctelia subrudecta is nitrogen tolerant. Heterodermia pseudospeciosa belong to physcioid lichen community and is toxi-tolerant species. The other seven lichen speciesact as bio-indicator of heavy metal air pollutants (iron, chromium, copper, zinc, lead and nickel (Table 1).

Table 1: Bio-Indicator Lichens of Sikandra Hill.

Sr. No.

Species

Family

Indicator & functional group

Remarks

1

Candelaria concolor (Dicks.) Arnold

Candelariaceae

Nitrophilous

Nitrophiles-- these species thrive in nutrient-enriched areas receiving N inputs from fertilizer application in agricultural areas or N emissions from power plants, automobile exhaust or industry (van Herk 1999). Among the most common in the NCR are Candelaria concolor,Flavoparmelia caperata,Flavopunctelia flaventior,Parmelia sulcata, Phaeophysciaorbicularis, Physcia aipolia,Physcia millegrana,Punctelia rudecta and P. subrudecta. These are the most common lichens in the NCR plots, and can be found even in park units nearest the center of Washington, D.C. (NAMA, NACE).

2

Dermatocarpon vellereum Zschacke

Verrucariaceae

Bio-indicator

Air pollution monitoring

3

Heterodermia pseudospeciosa (Kurok.) W.L. Culb

Physciaceae

Bio-indicator

Physcioid lichen community, Physcioid lichens are well known toxitolerant species employed for biomonitoring studies mostly in tropical regions.

4

Lecanora chlarotera Nyl

Lecanoraceae

Bio-indicator

Air pollution monitoring

5

Parmotrema praesorediosum (Nyl.) Hale

Parmeliaceae

Bio-indicator

Air pollution monitoring

6

Parmotrema tinctorum (Despr. ex Nyl.) Hale

Parmeliaceae

Bio-indicator

It is a bio-indicator of air pollution. It is especially sensitive to sulphur dioxide (SO 2) and has been threatened or even extinct in urban areas where the annual mean concentration of SO2 is more than 20 ppb (Sugiyama et al., 1976).

7

Phaeophyscia hispidula (Ach.) Essl

Physciaceae

Bio-indicator

Heavy metals were significantly increased due to exposure of pollutants

8

Physcia stellaris (L.) Nyl.

Physciaceae

Bio-indicator

Bio-indicator of air pollution

9

Punctelia subrudecta (Nyl.) Krog

Parmeliaceae

Nitrophilous tolerant

Bio-indicator of air pollution


CONCLUSION: During the present study, thirty species of lichen have been recorded from Skindra dhar of district Mandi. Out of these ten species of lichens act as bio-indicator. The result of this work will be useful for future studies of lichens to determine atmospheric pollution in the study area and other area as well.

ACKNOWLEDGEMENT: Authors are thankful to Chancellor, Career Point University Hamirpur for providing the necessary laboratory facilities.

REFERENCES:

1. Hawksworth D. L. and Rose F. (1976) Lichens as pollution monitors, (London, UK: Edward Arnold Ltd).

2. Stolte K., Mangis D., Doty R. and Tonnessen K. (1993) Lichens as Bioindicators of Air Quality (Fort Collins, Colorado: Rocky Mountain Forest and Range Experiment Station General Technical Report RM-224).

3. Ahmadjian, V. (1993) The Lichen Symbiosis, (New York: John Wiley and Sons).

4. Wolterbeek H. T., Garty J., Reis M. A. and Freitas M. C. (2003) Bio-monitors in use: lichens and metal air pollution. In: Markert B. A., Breure A. M. and Zechmeister H. G. (eds.) Bio-indicators and bio-monitors, (Oxford: Elsevier, 377-419).

5. Scheidegger C. and Werth S. (2009) Conservation strategies for lichens: Insights from population biology, Fungal Biology Reviews, 23, 55–66.

6. Loppi S. and Bonini I. (2000) Lichens and mosses as biomonitors of trace elements in areas with thermal springs and fumaroles activity (Mt. Amianta, central Italy), Chemo., 41, 1333–1136.

7. Upreti D. K., Divakar P. K. and Nayaka S. (2005) Commercial and ethnic use of lichens in India, Economic Botany, 59(3), 269-273.

8. Knops J. M. H., Nash T. H., Boucher V.L. and Schlesinger W. H. (1991) Mineral cycling and epiphytic lichens: implications at the ecosystem level, The Lichenologist, 23, 309-321.

9. Oyarún C. E., Godoy R. and Sepulveda A. (1998) Water and nutrient fluxes in a cool temperate rainforest at the Cordillera de la Costa in southern Chile, Hydrological Processes, 12, 1067-1077.

10. Forman R. T. T. (1975) Canopy lichens with blue-green algae: a nitrogen source in a Columbian rain forest, Ecology, 56, 1176-1184.

11. Becker V. E. (1980) Nitrogen fixing lichens in forests of the Southern Appalachian Mountains of North Carolina, The Bryologist, 83, 29-39.

12. Godoy R., Oyarún C. E. and Gerding V. (2001) Precipitation chemistry in deciduous and evergreen Nothofagus forests of southern Chile under low-deposition climate, Basic Applied Ecology, 2, 65-72.

13. Mueller G. M., Gerald F. B. and Mercedes S. F. (2004) Biodiversity of Fungi – Inventory and Monitoring Methods, (Burlington, USA: Elsevier Academic Press, 128-158).

14. Awasthi D. D. (2007) A Compandium of the Macrolichens from India, Nepal and Sri Lanka, (Dehra Dun, India: Bishen Singh Mahendra Pal Singh).

15. Goward T., McCune B. and Meidinger D. (1994a) The Lichens of British Columbia Illustrated Keys, part 1- Foliose and Suamulose Species, (British Columbia: Ministry of Forests, Research Program).

16. Goward T., McCune B. and Meidinger D. (1994a). The Lichens of British Columbia Illustrated Keys, part 2- Fruticose Species, (British Columbia: Ministry of Forests, Research Program).

17. White F. J. and James P. W. (1985). A new guide to the microchemical techniques for the identification of lichen substances, Bull. Brit. Lich. Soc., 57(Supplement), 1-41.

18. Thakur M. and Chander H. (2018) An Enumeration of Lichenized Fungi from Sikandra Dhar Region of District Mandi, Himachal Pradesh,J. Biol. Chem. Chron., 4(2), 104-116.